Deutrel Industries / Nu Science Corporation
155 West Avenue J-5
Lancaster, CA 93534

REPORT DATE: 07/08/99
DATE-INITIATED: 06/25/99
DATE-COMPLETED: 07/06/99
PROJECT #: 83454
REFERENCE #: 745-117
PO. #: N/A

SAMPLE DESCRIPTION:

ACCESSION #: 83454
SAMPLE: CELLFOOD
LOT.NO. ROM508
exp 02/2009

TEST-PERFORMED:
Validation of the Antimicrobial Preservative Effectiveness Test

BTS METHOD
M115.R3

TEST CONDITIONS:
Diluent - PBS with 1.0% polysorbate 80
Dilution Plated - 1:10
Media - Microbial Content Test Agar (Bacteria)
- Sabouraud Dextrose Agar (Yeast and Mold)

RESULTS:

MICROORGANISM		INOCULUM AVERAGE		MICROBIAL RECOVERY AVG.		PERCENT RECOVERY
Aspergillus niger		23		27		117.4
Candida albicans		26		18		69.2
Escherichia coli		115		68		59.1
Pseudomonas aeruginosa		47		46		97.9
Staphylococcus aureus		96		61		63.5

CONCLUSION:
The antimicrobial preservatives present in the sample can be neutralized under the test conditions described above